Title: Carbon catabolite repression of invertase during batch cultivations of Saccharomyces cerevisiae: the role of glucose, fructose, and mannose

标题：葡萄糖、果糖与甘露糖对酿酒酵母菌批培养中转化酶的碳分解产物抑制作用

Content/内容：

1) When Saccharomyces cerevisiae are grown on a mixture of glucose and another fermentable sugar such as sucrose, maltose or galactose, the metabolism is diauxic, i. e. glucose is metabolized first, whereas the other sugars are metabolized when glucose is exhausted.

酿酒酵母菌生长在葡萄糖与其他发酵糖类（如蔗糖、麦芽糖、半乳糖）混合物中，代谢呈双峰生长即首先是葡萄糖代谢；其次当葡萄糖耗竭时，其他糖类才开始代谢。

2) This phenomenon is a consequence of glucose геpression, or more generally, catabolite repression.

此现象是葡萄糖受抑制，或更多的是分解产物受抑制的结果。

3) Besides glucose, the hexoses fructose and mannose are generally also believed to trigger catabolite repression.

除了葡萄糖以外，一般还认为已糖类、果糖与甘露糖激发代谢抑制。

4) In this study, batch fermentations of S. cerevisiae in mixtures of sucrose and either glucose, fructose or mannose were performed.

在此研究中，进行的是在蔗糖与葡萄糖、果糖或甘解糖混合物中酿酒酵母菌的批发酵。

5) It was found that the utilization of sucrose is inhibited by concentrations of either glucose or fructose higher than 5 g/L, and thus that glucose and fructose are equally capable of exerting catabolite repression.

发现葡萄糖或果糖高于5g/L，抑制蔗糖利用，这样葡萄糖与果糖一样能发挥抑制分解产物的作用。

6) However, sucrose was found to be hydrolyzed to glucose and fructose, even when the mannose concentration was as high as 17g/L, indicating that mannose is not a repressing sugar.

然而，蔗糖水解为葡萄糖与果糖；甚至当甘露糖浓度高达17 g/L 时，也是如此，表明甘露糖无抑制作用。

7) It is suggested that the capability to trigger catabolite repression is connected to hexokinase P II, which is involved in the in vivo phosphorylation of glucose and fructose.

此提示激发分解产物受抑制作用与己糖磷酸激酶P II有关，而后者与体内葡萄糖与果糖的磷酸化有关。

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