Title: Amino acid utilization during cell growth and apoptosis induction

标题：在细胞生长与细胞凋亡诱导中的氨基酸利用

Content/内容：

1) The amino acid utilization between human promyelocytic leukemia (HL-60), human oral squamous carcinoma (HSC-2, HSC-4, NA), human salivary gland tumor (HSG) and rat neuron cells (PC-12) were compared, using amino acid analyzer.

应用氨基酸分析仪比较人早幼粒细胞白血病(HL-60)、人口腔鳞癌(HSC-2、HSC-4与NA)、人唾液腺肿瘤及大鼠神经细胞(PC-12)之间氨基酸的利用情况。

2) All these cells consumed four essential amino acids (valine, methionine, isoleucine, leucine), glutamine and arginine, whereas they produced glycine, alanine and ammonia, without significantly affecting threonine, tyrosine, phenylalanine, histidine or lysine concentration.

上述细胞类型均消耗4种基本氨基酸(缬氨酸、甲硫氨酸、异亮氨酸与亮氨酸)、谷氨酸与精氨酸，而它们均产生甘氨酸、丙氦酸与氨，但不明显影响苏氨酸与酪氨酸、苯丙氦酸、组氨酸与赖氨酸浓度。

3) Serine and glutamine utilization varied considerably from cell to cell.

丝氨酸与谷氨酰胺利用的细胞间变异很大。

4) HL-60 cells consumed serine and arginine at much higher rates than other cells.

HL-60细胞消耗丝氨酸与精氨酸的速度高于其他细胞。

5) Serine depletion accumulated the G1 arrested cells, and produced increasing numbers of the apoptotic cells.

丝氨酸耗竭累积于G1期阻止细胞，并且增加凋亡细胞数。

6) Supplementation of serine significantly extended the period of logarithmic cell growth.

补充丝氨酸明显延长细胞对数生长。

7) During apoptosisinduction of HL- 60 cells by dopamine, sodium ascorbate or sodium 5, 6-benzylidene-L-ascorbate, the oxidation of methionine to methionine sulfoxide was enhanced but the consumption of serine, glutamine and arginine was reduced.

在利用多巴胺、抗坏血酸钠或5,6-苯亚甲基-L-抗坏血酸钠诱导的HL-60细胞凋亡中，甲硫氨酸氧化成亚砜甲硫氨酸增加，但消耗丝氨酸、谷氨酰胺与精氨酸减少。

8) In the presence of HL-60 cells, the methionine oxidation was significantly inhibited, suggesting the antioxidant action of the cells.

在HL-60 细胞存在情况下，甲硫氨酸氧化明显受抑，提示细胞的抗氧化作用。

9) These present study suggests the importance of re-evaluation of culture condition for each cell line.

目前这些研究提示，重新评价每种细胞系培养条件的重要性。

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